Algae. 2011; 26(1): 79-86.
Culture study on the hybrid by interspecific crossing between Porphyra pseudolinearis and P. dentata (Bangiales, Rhodophyta), two dioecious species in culture
Nam-Gil Kim
Department of Marine Biology and Aquaculture / Institute of Marine Industry, Gyeongsang National University, Tongyeong 650-160, Korea
ABSTRACT
Physiological studies on the hybrid by crossing between two dioecious species, Porphyra pseudolinearis and P. dentata from Korea were conducted at constant temperatures (5, 10, 15, 20, and 25°C), at photon flux densities (10, 20, 40, and 80 μmol m-2 s-1) under photoperiods (14 L : 10 D and 10 L : 14 D). In the hybrid, higher growth of conchocelis was observed at 20 and 40 μmol m-2 s-1 under 14 L : 10 D. Conchosporangial branches were produced under 10-80 μmol m-2 s-1 at only 25°C, and were abundant when the conchocelis was cultured under 10 L : 14 D. Foliose thalli of the hybrid grew well at the conditions of 10-20°C, 10 L : 14 D and 15-20°C, 14 L : 10 D. The foliose thalli grew very slowly at 5°C and 30°C, respectively. No archeospores were observed at any culture conditions. Spermatangial and zygotosporangial sori were formed at the marginal portion of mature thallus. Zygotospores from the hybrid were released at 10-20°C under both photoperiods, and gave rise to form conchocelis filament. Monoecious thalli were observed at 10°C under 14 L : 10 D. Neither monospores nor protothalli were produced from the conchocelis in culture.
Keywords :
Bangiales; hybrid; interspecific crossing; physiology; Porphyra dentata; Porphyra pseudolinearis; Rhodophyta
