Algae. 2002; 17(3): 153-159.
Phylogenetic Relationship of Microcystis (Cyanophyceae) Based on Partial 16S rRNA Gene Sequences in Korea
Kim Jong-In, Jong Hun Lim, Jae Wan Lee, Hae-Bok Lee
Department of Life Science, Chongju University, ChungCheongBuk-Do Institute of Health & Environmental Research
Partial 16S rRNA gene sequences of seven cyanophycean strains from the National Institute of Environmental Research of Korea - Microcystis aeruginosa, M. aeruginosa f. aeruginosa, M. ichthyoblabe, M. viridis, Anabaena flos-aquae, and Oscillatoria sancta - were analyzed and the phylogenetic relationship of Microcystis among Cyanophyceae were evaluated. Based on the sequence analysis results, Microcystis is monophyletic, the clade of which supported 100% bootstrap trees, and distinguished clearly from the other taxa. Therefore, the partial 16S rRNA gene sequences can be a useful and efficient tool for distinguishing Microcystis from other cyanophycean genera without axenic culture or cloning.
Keywords : Cyanophyceae, 16S rRNA, Microcystis, phylogeny