Algae. 1998; 13(1): 157-163.

Isolation and Characterization of a cDNA Encoding 40S Ribosomal Protein S8 from a Red Alga, Griffithsia japonica (Ceramiales, Rhodophyta)

Yoo-Kyung Lee, Choo-Bong Hong, Nam-Kyu Lim, Jae-Seong So, In-Kyu Lee

Department of Biology, and Institute for Molecular Biology and Genetics, Seoul National University, Department of Biological Engineering, Inha University, Department of Biology, Seoul National University

A cDNA clone, GjFW-1 (Griffithsia japonica Female Weak-1) was isolated by differential screening of a gametophyte-specific cDNA library of a red alga, G. japonica. The transcript corresponding to GjFW-1 was detected strongly in the vegetative thalli, male gametophytes and tetrasporophytes, but very weakly in the female gametophytes. The nucleotide sequence of GjFW-1 was identified as an open reading frame encoding 204 amino acid residues, which shared 52-63% homology with the previously reported 40S ribosomal protein S8 of other organisms. This is the first report on algal S8 protein cDNA clone.

Keywords : 40S ribosomal protein S8, GjFW-1(japonica Female Weak-1), Griffithsia japonica, red algae, sex-related cDNA