Algae. 2009; 24(2): 105-110.
Detection of Fish Killing Dinoflagellates Cochlodinium polykrikoides and Karlodinium veneficum (Dinophyceae) in the East China Sea by Real-time PCR
Tae-Gyu Park*, Yang-Soon Kang, Young-Tae Park, Heon-Meen Bae and Yoon Lee
Marine Ecology Research Division, National Fisheries Research and Development Institute, Busan 619-705, Korea
The rDNAs of fish-killing dinoflagellates Cochlodinium polykrikoides and Karlodinium veneficum were detected from the East China Sea by species-specific real-time PCR probes. Sequence analyses using the partial ITS sequences from the real-time PCR products showed identical sequences with C. polykrikoides and K. veneficum, respectively and low expectation values (E-value) of less than 1e-5 suggesting the presence of these organisms in the East China Sea shelf water that flows into the Tsushima Strait and the Yellow Sea.
Cochlodinium polykrikoides, East China Sea, Karlodinium veneficum, real-time PCR, red tide