Algae. 2000; 15(3): 143-147.

Mitochondrial Dynamics in Red Algae. 1. Monospore Germination in Audouinella botryocarpa (Acrochaetiales)

David J. Garbary, Pei Zuchang

Mitochondrial distribution was examined during monospore germination in Audouinella botryocarpa (Harvey) Woelkerling using fluorescence microscopy and staining with DiOC sub(6) (3) and DASPEI. Mitochondria varied in shape from spherical to ovoid or irregular. In released monospores mitochondria were evenly distributed. During germination many mitochondria became concentrated where the single germination tube emerged, and large mitochondrial complexes developed. This dense aggregation of mitochondria became associated with tip growth of the germ tube, and was retained through at least several apical cell divisions of the sporeling. In addition, many mitochondria remained in the spore and formed large complexes associated with the transverse wall of the developing filament. They were retained during the apical cell division of the sporeling and subsequently declined in size and number.