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Algae > Volume 38(2); 2023 > Article
Hajiya Hasan, Al-Bader, Woodward, Antóny, Ong, Peters, and Küpper: Assessment of the macroalgal diversity of Kuwait by using the Germling Emergence Method

ABSTRACT

Cryptic stages of diverse macroalgae present in natural substrata, “the bank of microscopic forms”, were isolated into clonal cultures and identified based on both morphological characteristics and DNA barcoding. Approximately 120 clonal isolates from 308 natural substratum samples were collected from the entire coastline of Kuwait. Amongst these isolates, 77 (64%) were identified through DNA barcoding using the nuclear ribosomal small subunit, RuBisCO spacer (ITS2, tufa, rbcL, psaA, and psbA) and sequencing. Twenty-six isolates (34%) were identified in the division Chlorophyta, 18 (23%) as Phaeophyceae, and 33 (43%) as Rhodophyta. For all DNA sequences in this study, species-level cut off applied was ≥98% homology which depend entirely on the markers used. Three putative new records of Chlorophyta new for the Arabian Gulf were made: Cladophora laetevirens (Dillwyn) Kützing, Ulva torta (Mertens) Trevisan and Ulvella leptochaete (Huber) R. Nielsen, C. J. O′Kelly & B. Wysor in Nielsen, while Cladophora gracilis Kützing and Ulva ohnoi M. Hiraoka & S. Shimada are new records for Kuwait. For Phaeophyceae, Ectocarpus subulatus Kützing and Elachista stellaris Areschoug were new records for the Gulf and Kuwait. In the Rhodophyta, Acrochaetium secundatum (Lyngbye) Nägeli in Nägeli & Cramer, Ceramium affine Setchell & N. L. Gardner, Gelidium pusillum var. pakistanicum Afaq-Husain & Shameel and Dasya caraibica Børgesen are new records for the Gulf and Kuwait, while the red alga Stylonema alsidii (Zanardini) K. Drew is a new record for Kuwait. Several isolates identified corresponded to genera not previously reported in Kuwait and / or the Arabian Gulf, such as Porphyrostromium Trevisan, a new genus from the Bangiales, and two unidentified species for the Planophilaceae Škaloud & Leliaert. The isolates cultivated from substrata enhance understanding of the marine macroalgal diversity in the region and confirmed that the Germling Emergence Method is suitable for determining the actual diversity of a given study area through isolation from cryptic life-history phases.

INTRODUCTION

Globally, numerous shorelines are yet to be explored for benthic marine algal diversity (Wynne et al. 2020). Marine algae are important shelter or habitats for sessile - micro- and macro-organisms and are the basis of food webs in marine ecosystems (Wolf 2012, Macreadie et al. 2017). However, only approximately 10% of algal diversity have been discovered and formally described, with a lack of knowledge especially in remote regions (De Vargas et al. 2015, Bartolo et al. 2020). Seaweeds produce reproductive cells or multicellular propagules at maturity, many of which will attach to available substrata and, after a period of time, form a “bank of algal microscopic forms” (Hoffmann and Santelices 1991, Peters et al. 2015, Schoenrock et al. 2021). However, these cryptic life forms are difficult to capture and characterize.
In the last 20–30 years, the development of in vitro culture techniques for isolates from incubated natural substrata, such as small pebbles and sand grains collected during fieldwork or diving surveys, has helped in the investigation of macroalgal microscopic stages from remote and under sampled areas (Ramírez and Müller 1991, Müller and Ramírez 1994, Peters et al. 2015). The Germling Emergence (GE) technique has the potential to discover cryptic species and new records of macroalgal taxa by a combination of morphological characterization with DNA barcoding. Comparing short DNA barcodes with available reference sequences in public databases can lead to accurate identification of cultured microscopic species or microstages that can be difficult to classify morphologically (Peters et al. 2015, Bartolo et al. 2020). For example, Zuccarello et al. (2011) and West et al. (2012) reviewed the order Erythropeltidales (Rhodophyta) using a combination of the GE method and molecular data that enabled new records and discoveries of macroalgal taxa around the world. These discoveries included, for example, a novel Desmarestia species and a number of Ectocarpales species from the Canadian Arctic (Küpper et al. 2016), two novel members of the Pelagophyceae, Sungminbooa kuepperi R. A. Andersen & B. Melkonian from the Beagle Channel, Tierra del Fuego, and Chrysoreinhardia muelleri M. Melkonian & R. A. Andersen from the Falklands (Han et al. 2018). Peters et al. (2015) used the GE method combined with 5′-cytochrome oxidase (COI) barcoding to detect cryptic diversity (including three putative new species) within Ectocarpales collected from different geographical regions. Additionally, in circalittoral waters (24 m depth) of the Mediterranean Sea, the GE method succeeded in isolating the minute benthic multicellular alga Schizocladia ischiensis; thus, this method appears to reveal benthic algae even when small in size (Rizouli et al. 2020). In another study, isolates of the genus Ectocarpus and associated bacterial strains from Hopkins River in Australia, obtained using the GE method, provided the possibility to study Ectocarpus adaptations to abiotic parameters - especially low salinity and the interactions of the alga with the endogenous microbiome (Dittami et al. 2020b). Recently, the GE method enabled the description of a new species of red alga from the Celebes Sea, Hypoglossum sabahense (Wynne et al. 2020).
The aim of the work presented here was to identify algal isolates from a collection of microscopic forms from different locations in Kuwait, obtained using the GE technique. A few additional algal cultures were obtained from fragments or reproductive cells of macroscopic algae. Molecular identification and establishment of gene phylogenies were carried out using commonly employed markers, for which there were sufficient reference sequences in public databases. The mitochondrial COI is a useful barcode marker since it discriminates well between species and it has become a preferred locus in DNA barcoding of brown and red macroalgae (Saunders and McDevit 2013). The plastid-encoded large and small subunits of ribulose-1,5-bisphosphate carboxylase (rbcL and rbcS, respectively) are separated by a spacer, which is variable in length (Destombe and Douglas 1991). rbcL is also among the preferred loci for phylogenetic studies and DNA barcoding of brown macroalgae (Siemer et al. 1998, Saunders and McDevit 2013), although it is slightly less discriminatory than COI between species of Phaeophyceae. The RuBisCO spacer is highly variable and cannot serve for phylogenetic analyses because of limited alignability across species and genera. Sequences of the spacer, however, are occasionally published together with rbcL sequences and can be used as barcodes for the identification of species (Siemer et al. 1998).

MATERIALS AND METHODS

Field sites, specimens, and laboratory culture

Samples were collected during late winter and spring (February and April 2019), from 38 sampling points (sites) in 12 localities representing the different maritime regions from Northern and Southern Provinces in Kuwait including offshore islands (Fig. 1). Of the 308 total individual samples, 261 (85%) were abiotic (small fragments of rock, or pebbles) while 47 (15%) were biotic substrata (fragments of subtidal macroflora, such as seagrass leaves and Sargassum blades). Generally, samples were collected from each sector at 3 transects; upper (closest to the shore) middle, lower intertidal zone and rocky shore at each location during low tide. Furthermore, substratum samples were collected from several of the islands of Kuwait by SCUBA diving in the shallow subtidal zone around Failaka and Umm al-Maradim (15 m), Qaruh (6 m), and Kubbar (10–35 m) (Supplementary Table S1). Also, the sampling were covered various distances from brine outfalls at typical two desalination plants, namely; Doha East (DE) and Al-Zour South (ZS). Triplicate samples of natural substrata were placed in 15 mL FALCON tubes previously filled with 10 mL heat-sterilized sea water, before transport to the Bezhin Rosko Laboratory of AFP (Santec, France). There, the contents of each tube were transferred to 55 mm diameter Petri dishes containing 8 mL Provasoli-enriched natural autoclaved sea water culture medium (Provasoli 1966, Starr and Zeikus 1993, Tarakhovskaya et al. 2012) and incubated at 15°C in natural daylight at a north-facing window for 2–6 weeks. In order to prevent diatom growth, germanium dioxide (GeO2, 3–4 mg L−1) was added to the medium (Peters et al. 2015). After three to four weeks, unialgal isolates were obtained from the dishes by cutting accessible parts of germlings with a sharp, freshly snapped, glass Pasteur pipet tip and transferred into fresh culture medium without GeO2 (Lewin 1966). The laboratory culture followed standard methods (Coelho et al. 2012), with monthly serial transfer. The temperature was maintained at a constant 25 ± 1°C in a CO2 incubator (240 L MIR-253-CFC FREE; Sanyo, UK), irradiance was by dimmed natural light (less than 40 μmol m−2 s−1 photon fluence rate) measured with a Skye Instruments SKP 200 light meter (Skye Instruments Ltd., Llandrindod Wells, Wales, UK) and daylengths were based on the natural change. To minimize duplicate isolation of the same species, multiple isolates were made from each dish only when algae exhibited different morphologies. Strains that were not single cultures after a further two months of cultivation were excluded from subsequent analyses. The February and April isolates were deposited in collections at the University of Aberdeen, Scotland, UK, in the Bezhin Rosko Laboratory (France) and in the Algal Culture Unit (KUAC) at Kuwait University, Kuwait.

Molecular work and phylogenetic analyses

DNA was extracted from 5–15 mg wet weight of algal cultures using CTAB, and the GENEJET Plant Genomic DNA Purification Kit (Thermo Scientific, Vilnius, Lithuania) according to the manufacturer’s protocol (Gachon et al. 2009). Extracted DNA from the unialgal isolates was amplified by polymerase chain reaction (PCR) and sequenced. First, the nuclear ribosomal small subunit nrSSU locus was used to confirm that the extracted DNA was suitable for amplification. However, this marker did not discriminate sufficiently between species, and barcode markers with higher resolution were used for final identification (Bartolo et al. 2020). Therefore, several markers were used together with the nrSSU locus to obtain species-level identification. Internal transcribed spacer 1 (ITS1), internal transcribed spacer 2 (ITS2), and plastid elongation factor (tufA) locus used for Chlorophyta, the partial mitochondrial gene regions (5’COI) to examine Phaeophyceae and plastid-encoded psaA, psbA gene and plastid locus, such as RuBisCO spacer region rbcL were used for Rhodophyta (Table 1). Two markers were used for isolates of Chlorophyta, the plastid-encoded DNA barcode marker elongation factor tufa and the nuclear ITS2. tufA has been used to discriminate amongst green algal species (Kirkendale et al. 2013) and in an evaluation of barcode markers for marine green macroalgae (Saunders and Kucera 2010), this primer had high levels of discriminatory power between species. The second marker, ITS2, has also been widely used in species-level phylogenetic studies of green algae (Lawton et al. 2013).
PCR products were Sanger sequenced by a commercial service (Source Biosciences, Cambridge, UK). Consensus sequences were aligned using the software BioEdit Editor (Hall et al. 2010) and sequences had a higher homology than 98% identity were compared to published data by using NCBI BLAST searches (http://www.ncbi.nlm.nig.gov) (Altschul et al. 1997). Sequence alignments were done by using the Multiple Sequence Comparison by Log-Expectation (MUSCLE) statistical method with the software MEGA X 11.0.11 (http://www.megasoftware.net) (Edgar 2004, Kumar et al. 2018).

RESULTS

Field collections and algal isolates

We obtained 345 unialgal macroalgal clones from 308 substrate samples (i.e., an average of 1.12 strains per sample). One hundred and twenty clonal isolates were included in this study. Out of these 120 clones, 84 (70%) were obtained using the GE method, while 35 (29%) were isolated directly from macroscopic thalli collected in the field and 1 strain (0.8%) was obtained in culture by fertilization of fertile cells in vitro (settled zoids) (Supplementary Table S1). Amongst the 120 isolates, 87 (72%) strains were collected in the vicinity of the DE and ZS desalination plants (April 2019) and the other 33 (28%) were collected from different sites on the Kuwait coast (February 2019).

DNA sequences of isolates obtained by Germling Emergence

Of the final 120 isolates selected for molecular identification, the majority were Rhodophyta (n = 51/120 strains; 42%), with the most commonly identified species belonging to the Erythrotrichiaceae G. M. Smith, especially Erythrotrichia Areschoug sp., and Chlorophyta (n = 45/120 strains; 38%) were present in smaller numbers, with many independent replicates of Ulva tepida Y. Masakiyo & S. Shimada (KT374006; described from Australia by Masakiyo and Shimada 2014). Phaeophyceae (n = 24/120 strains; 20%) were present in still smaller numbers, the most frequently identified species being a close relative of Feldmannia mitchelliae (Harvey) H. -S. Kim sensu lato (s.I) (AB302306; described from Japan by Tanaka et al. 2010). Only for 77 out of the 120 isolates, PCR-friendly DNA could be extracted (64%). Of the remaining 43 strains, 17 showed weak PCR amplification which suggested an imperfect match of PCR primers and 14 strains were only identified morphologically (Ulva tepida), but for 12 strains no PCR amplification was achieved.
Based on 130 DNA sequences, using the nuclear ITS2 locus and the chloroplast-encoded tufA primers for Chlorophyta, 26 of the 77 extracted isolates yielding PCR-friendly DNA (corresponding to 34%, representing 17 species) were identified. In the Phaeophyceae, several sequences were obtained of chloroplast psaA, mitochondrial COI, rbcL and RuBisCO spacer (18 of the 77 extracted isolates yielding PCR-friendly DNA, i.e., 23%, representing 14 species). In the Rhodophyta, rbcL and psbA sequences are used to identify 33 out of the 77 isolates with PCR-friendly DNA (43% of the total, representing 26 species). Based on the barcoding results (Supplementary Table S2), 20 sequences supported species-level identity (6 green, 7 brown, and 7 red algae, respectively), 21 enabled genus-level identification, and 14 clustered with higher-level taxonomic entities in each phylum. All sequences were deposited in GenBank/NCBI (outlined in Supplementary Table S2).

DISCUSSION

In the present work, the GE method with subsequent DNA barcoding to characterize cryptic macroalgal diversity was applied for the first time to seaweeds sampled in the Arabian Gulf region. While this work revealed a number of novel species for the region, the isolated taxa probably underrepresent the diversity of cryptic stages present in the field, as GE is biased towards rapidly growing and proliferating, early successional species.

Diversity and distribution of GE isolates in Kuwait

In the present work, a total of 57 species of algae (Table 2) were identified from 77 DNA-sequenced isolates sampled on the coast of Kuwait, identified morphologically and underpinned by molecular information (Supplementary Table S2). In addition, genetic analyses revealed that the GE isolates sequences were not uniformly distributed among the 3 major macroalgal phyla. Among 120 unialgal isolates, the results revealed 45 isolates representing 17 species of Chlorophyta including Ulvaceae J. V. Lamouroux ex Dumortier, Ulvellaceae Schmidle, Bryopsidaceae Bory, Cladophoraceae Wille, and Planophilaceae Škaloud & Leliaert. Twenty-four isolates representing 14 species in the Sphacelariaceae Decaisne, Acinetosporaceae G. Hamel ex Feldmann, Chordariaceae Greville, Ectocarpaceae C. Agardh, Scytosiphonaceae Farlow, and Bachelotiaceae T. Silberfeld, M.-F. Racault, R. L. Fletcher, A. F. Peters, F. Rousseau & B. de Reviers for Phaeophyceae. According to Peters et al. (2015), species of Chordariaceae are small, with some lacking a macroscopic stage, making it particularly difficult to distinguish species based solely on morphological features. Thus, DNA barcoding has a key role in facilitating species identification in this family. Finally, 51/120 strains coveting Stylonemataceae K. M. Drew, Acrochaetiaceae Melchior, Erythrotrichiaceae G. M. Smith, Spyridiaceae De Toni, Gelidiaceae Kützing, Delesseriaceae Bory, Ceramiaceae Dumortier, Callithamniaceae Kützing, Rhodomelaceae Horaninow, and Bangiaceae Duby represent 26 species of Rhodophyta (Table 2). Overall, 95/120 (79%) of the strains were collected from the Southern Province, including Fintas, whereas 25 (21%) strains were collected from the Northern Province, including Boubiyan Island.
The proportion of Phaeophyceae (n = 18/77 isolates; 23%) was rather small among the initial cultures. Also, species richness among the brown algae isolated here was lower than for the other identified phyla. Amongst the isolates of filamentous Ectocarpales Bessey in the data obtained, Feldmannia Hamel is less well studied than the genus Ectocarpus Lyngbye (Peters et al. 2015), with few reference sequences available in GenBank when using the locus RuBisCO spacer / rbcL region (amplified with primers rbcLP2F, rbcS 952R or rbcL RH3F and rbcS139R). The results obtained in this study showed that the strain K23 [K19-4-130-1] using the 3′-rbcL plus RuBisCO spacer (using primers rbcL1273F, rbcS 139R) was identical (100% homology in both rbcL and RuBisCO spacer) to a sequence for Ectocarpus subulatus from Port Aransas, Texas, USA (accession No. U38750), indicating that this strain belonged to the same species. Originally identified as E. siliculosus, the Ectocarpus from Port Aransas is now regarded as a different species, E. subulatus, which is, in ecophysiological terms, the most low salinity-tolerant species of the genus (Dittami et al. 2020a, 2020b). Port Aransas harbors the southernmost population of Ectocarpus known in North America, with a large temperature amplitude of 17°C between winter (13°) and summer (30°) (Bolton 1983). It is interesting that the same Ectocarpus species appears to be present in Kuwait, where the summer temperature is even higher. Further analyses of the rbcL gene sequences showed that isolates (K72 [K19-4-123-1] and K84 [K19-2-72-5]) had closest matches (98.70 and 98.16% homology) to sequences of Sphacelaria tribuloides Meneghin from the Netherlands (AJ287892) and Okinawa (AJ287891), respectively. The RuBisCO spacer of K84 (262 bp in length) showed 91.6% homology with that of S. tribuloides from Okinawa (AJ287947). Taken together, the results from rbcL and RuBisCO spacer suggest that the material from Kuwait was a closely related species, possibly, to the morphologically similar Sphacelaria novae-hollandiae Sonder, for which no published sequences are available. According to Al-Yamani et al. (2014), both S. tribuloides and S. novae-hollandiae occur in Kuwait. However, for example, Sargassum and Padina sporophytes were not encountered in cultures starting from substrates despite a clear abundance in the field. These algae have no long-lived microstages (microscopic gametophytes or sporophytes) in their life cycle (Peters et al. 2015).
In Kuwait, at the Northern province sites; Boubiyan, Sabiya, Failaka, and Doha, respectively, had less algal diversity than the Southern provience. Three brown algal isolates, Ectocarpus subulatus (K23), Colpomenia sinuosa Derbes et Solier (K87) - Sphacelaria Lyngbye sp. (K71), plus two red taxa, Stylonema alsidii (Zanardini) K. Drew (K12) which has a world-wide geographic distribution (Zuccarello et al. 2011), which had previously been reported from Kuwait (Al-Yamani et al. 2014), and Spyridia Harvey sp. (K66) were identified (Table 3). However, these are clearly adapted to unstable substrates. The limited occurrence of Phaeophyceae and Rhodophyta the Northern Province (Doha) can also be correlated with the muddy substrates there (Jones 1986) which suggests that this area may lack the microstages of taxa in these phyla, compared with other species that were obviously present in this area and that were mostly Chlorophyta. The Northern Province including the Doha site (Table 3) is characterized by shallow, extensive intertidal mud flats with turbid water and semi-enclosed bodies of water with very limited exchange (65 days) to the open sea (Pokavanich and Alosairi 2014) which is typical for Kuwait Bay (Al-Mutairi et al. 2014), compounded by a scarcity of solid, rocky substrates (Alghunaim et al. 2019). The soft nature of these substrates can also explain why the Northern Province is mostly unsuitable for the attachment and growth of several macroalgae particularly large brown macroalgae, such as Sargassum C. Agardh, except for some taxa with small thalli such as Feldmannia sp., which agrees with our observations.
In most temperate coastal waters, macroalgal growth and diversity are directly controlled by nutrient availability (Pedersen et al. 2010, Martínez et al. 2012). The marine environment of Kuwait is also strongly influenced by the discharge of the Shatt Al-Arab estuary with a maximum peak of discharging fresh water from March to July (Al-Said et al. 2017). This freshwater input is associated with a higher concentrations of nutrients, resulting in increased productivity and abundance of green algae in the Doha area. Also, the green algal taxa recorded inhabited calm waters (tidal pools), which were plentiful in the Doha area during winter and early spring where there are many flat, intertidal rock platforms with low current waves, allowing the development of a stable community with higher diversity of green algae (Prathep et al. 2007). Nevertheless, nutrient enrichment and low wave action (Nishihara and Terada 2010) can, therefore, increase the growth of opportunistic seaweeds like green algae characteristically found at Al-Doha, represented in this study by Ulva Linnaeus spp. and members of the Ulvellaceae. It seems that the Ulva species are among the fastest growing macroalgae under conditions of high nutrient and light availability (Phillips and Hurd 2003). Competition among thalli of green algae for space at Doha is intense, in particular when light levels increase in spring and Ulva species generally have high levels of desiccation resistance (Nybakken 1993).
The Chlorophyta recorded in this work (n = 26/77, 34%) mostly have small holdfasts, are smaller and lighter in weight, and are tolerant to the prevailing turbidity and trophic levels compared to Phaeophyceae (Al-Hasan and Jones 1989, Uddin et al. 2011). Furthermore, as Sargassum species are very sensitive to environmental perturbations, substrate type and slope (Fatemi et al. 2012), they are not encountered in the Doha area. A limited number of more tolerant species, however, such as Feldmannia sp. and Iyengaria Børgesen sp., are encountered at Doha. There is some evidence that, over the last two decades, algal diversity on the Doha coast has decreased (Dhia Al-Bader, personal communication / unpublished results).
The Southern Province was the richest area in terms of diversity of algal isolates (95 strains/120; 79%), especially at Fintas and Al-Zour (Table 3). The more benign physical conditions compared to the northern areas of Kuwait probably result in an increase in richness in the “bank” of microscopic stages on this sandy and rocky coast. This finding may be attributed to several factors, such as the lesser anthropogenic impacts on the open sea environment in the south, along with the higher hydrodynamic energy with low turbidity in this region (2 m tidal range) (Al-Yamani et al. 2004). One of the most important factors in species distribution and abundance in tidal areas is exposure to wave action and overall hydrodynamism (Mayakun and Prathep 2005, Nishihara and Terada 2010). According to Al-Yamani et al. (2004), the scattered rocks available on the beaches of this province are suitable for seaweed attachment by holdfasts, resulting in diverse communities of algae. Microscopic stages will inhabit rock surfaces, pebbles and shell fragments, creating a more diverse range of isolates compared to Doha. This finding was emphasized by Al-Yamani et al. (2014) and Al-Hasan and Jones (1989), who also mention this pattern in their previous publications about the macroalgal flora of Kuwait. This is also consistent with Uddin et al. (2011), who mentioned that the southern waters of Kuwait have coral reefs that can also support rich biodiversity of marine macrophytes. Al-Zour is characterized by a rocky shore and overall is a protected and sheltered area, therefore a high diversity of seaweeds, especially among the Rhodophyta, was not surprising. Overall, in the present study, the Rhodophyta were the phylum with the highest species richness compared to the green and brown phyla among seaweeds isolated and identified in this study, constituting 42% (n = 51/120) of the total algal isolates, representing 26 species. These observations are consistent with several previous reports (Silva et al. 1996, Price et al. 2006, John 2012, John and Al-Thani 2014) who suggested that among a total of approximately 282 benthic marine algal species in the Arabian Gulf, the most diverse were the Rhodophyta (147 spp.).
A further exploration of the macroalgal diversity on the coast of Kuwait will likely reveal greater diversity, especially of smaller epiphytic algal species with short life cycles, as the time period covered in this work encompassed only late winter (February 2019) and spring (April 2019). The GE method was clearly a good tool to investigate cryptic macroalgal floral diversity. Compared to environmental sequencing (metabarcoding) it offers the advantage of providing access to a plethora of biological information such as morphology, life cycle, physiology and biochemistry. This method has substantially helped revealing a diversity in the local Kuwaiti waters that was not previously known. This study has revealed several new records of species and genera, including noteworthy are new records of Porphyrostromium, a new genus from the Bangiales namely Kuwaitiella rubra gen. et sp. nov., and two unclear species for Planophilaceae. The data obtained in this present study will also help to predict the future of algal diversity and floristic composition under ongoing and predicted climate change effects impacting this already harsh environment.

ACKNOWLEDGEMENTS

The present work formed part of the first author’s PhD thesis ‘Macroalgal biodiversity of Kuwait, with special emphasis on the vicinity of desalination plants’. We acknowledge Dr. Hedda Weitz (University of Aberdeen) for providing help in the laboratory and from Ioanna Kosma (University of the Aegean) and Andreas Henkel (Kuwait University) for diving and logistics support during the expedition to Kuwait. We acknowledge the funding received to support this work from the Marine Alliance for Science and Technology (grant reference HR09011) to FCK and Kuwait Foundation for the Advancement of Science (KFAS; grant number PR17125L18) to DA. To Mr. Yusuf Buhadi from the department of Marine Sciences at Kuwait University for his help in the field work and to Mrs. Nisha V. S. Vadakkhancheril for photography.

CONFLICTS OF INTEREST

The authors declare that they have no potential conflicts of interest.

SUPPLEMENTARY MATERIALS

Supplementary Table S1
Identification, habitat, and collection sites of the 120 algal cultures in Kuwait (https://www.e-algae.org).
algae-2023-38-2-127-Supplementary-Table-1.pdf
Supplementary Table S2
List of isolated algal strains collected from Kuwait, grouped according to the closest sequence match in GenBank (https://www.e-algae.org)
algae-2023-38-2-127-Supplementary-Table-2.pdf
Supplementary Fig. S1
(A–C) The identification of the species was based upon molecular analyses of in vitro algal cultures (under laboratory conditions with specific light : dark time period)—mostly in a microscopic stage and not always similar morphologically to growth in the natural environment (https://www.e-algae.org).
algae-2023-38-2-127-Supplementary-Fig-1.pdf

Fig. 1
Localities and sites sampled. (A) General location of Kuwait in the northwestern of the Arabian Gulf. (B) Map of Kuwait represents 1–12 sampling points including shoreline and off shore Islands namely; Bubiyan, Sabiya, Failaka, Doha, Kuwait City Sharq, Fintas, Kubbar, Qaruh, Al- Zour, Al-Khiran, Umm Al-Maradim, and Nuwaiseeb, respectively. Subset of maps showing sample locations at (C) Doha 4 and (D) Al-Zour 9 and Al- Khiran 10 sites (GIS maps produced using ArcGIS software; National Geographic, Esri, Garmin, USA).
algae-2023-38-2-127f1.jpg
Table 1
List of oligonucleotide primers of amplification used to amplify different fragments of DNA from genomes of algal isolates
Locus Target DNA Primer name Sequence (5′ to 3′) Fragment size (bp) Reference
Nuclear nrSSU NS1F
NS4R
GTAGTCATATGCTTGTCTC
CTTCCGTCAATTCCTTTAAG
1,100 White et al. (1990)
ITS1 AFP4LF
5.8S1R
CAATTATTGATCTTGAACGAGG
TGATGATTCACTGGATTCTG
1,056 Peters et al. (2004)
ITS2 KP5F
KG4R
ACAACGATGAAGAACGCAG
CTTTTCCTCCGCTTAGTTATATG
600 Lane et al. (2006), Hodge et al. (2010)
Plastid elongation factor tufA tufAF
tufAR
TGAAACAGAAMAWCGTCATTATGC
CCTTCNCGAATMGCRAAWCGC
850 Famà et al. (2002)
Chloroplast psbA psbA1F
psbA1R
ATGACTGCTACTTTAGAAAGACG
GCTAAATCTARWGGGAAGTTGTG
1,000 Yoon et al. (2002)
psaA psaA130F
psaA970R
AACWACWACTTGGATTTGGAA
GCYTCTARAATYTCTTTCA
900 Yoon et al. (2002)
Mitochondrial 5′COI GazF2
GazR2
CCAACCAYAAAGATATWGGTAC
GGATGACCAAARAACCAAA
~700 Saunders (2005)
Plastid RuBisCO spacer RH3F
rbcS139R
AGCCCCATCACGATGCAGTT
AGACCCCATAATTCCCAATA
1,000 Peters and Ramírez (2001)
rbcLP2F
rbcS952R
GAWCGRACTCGAWTWAAAAGTG
CATACGCATCCATTTACA
950 Kawai et al. (2007)
rbcL1273F
rbcS139R
GTGCGACAGCTAACCGTG
AGACCCCATAATTCCCAATA
~400–600 Peters and Ramírez (2001)
rbcL KitoF1
JrSR
ATGTCTCAATCCGTAGAATCA
AAGCCCCTTGTGTTAGTCTCAC
1,600 Kunimoto et al. (1999), Broom et al. (2010)
Table 2
Species names and authorities for Chlorophyta, Phaeophyceae, and Rhodophyta isolated from Kuwait (February–April 2019) based on DNA barcoding results
No. Strains ID Present study Synonyms Comments
Chlorophyta
 Cladophoraceae
 1 K56 Cladophora laeteviren (Dillwyn) Kützing Conferva laetevirens Dillwyn New record for Kuwait & the Gulf based on molecular analysis
 2 K57 Cladophora gracilis Kützing Kokabi and Yousefzadi (2015) mentioned this sp. in Gulf, it is a new based on molecular analysis for Kuwait
 3 K73, K74 Chaetomorpha Kützing sp. Al-Yamani et al. (2014) mentioned Ch. crassa (C. Agardh) Kützing, Ch. indica (Kützing) Kützing, Ch. linum (O. F. Müller) Kützing, first record underpinned by molecular information in Kuwait
 4 K58 Rhizoclonium Kützing sp. Al-Yamani et al. (2014) mentioned R. riparium (Roth) Harvey and R. tortuosum (Dillwyn) Kützing, first underpinned by molecular analysis in Kuwait
 Ulvaceae
 5 K1, 3, 4, 5, 25, 51, 52, 54 Ulva tepida Y. Masakiyo & S. Shimada Ulva paschima F. Bast Recorded based on molecular information by Pirian et al. (2016) for the Gulf and by Al-Adilah et al. (2021) for Kuwait
 6 K7 Ulva torta (Mertens) Trevisan Conferva torta Mertens New record for Kuwait & Gulf based on molecular analysis
 7 K55 Ulva ohnoi M. Hiraoka & S. Shimada First record for the Gulf, supported by molecular data showing homology described by Pirian et al. (2016)
 Ulvellaceae
 8 K45 Ulvellaceae Schmidle sp. Kokabi and Yousefzadi (2015) mentioned U. viridis in Gulf, but our strain is different and a new record for Kuwait, with molecular support
 9 K42, K43, K46 Ulvellaceae Schmidle sp.
 10 K50 Ulvella leptochaete (Huber) R. Nielsen, C. J. O’Kelly & B. Wysor in Nielsen Endoderma leptochaete Huber New record for Kuwait & the Gulf, based on molecular information
 11 K39, K40, K44 Ulvella P. Crouan & H. Crouan sp.
 Planophilaceae
 12 K38, K41 Planophilaceae Škaloud & Leliaert sp. John and Al-Thani (2014) mentioned P. dendroides (P. L. Crouan & H. M. Crouan) Batters in the Gulf; this family is a new record for Kuwait
 Bryopsidaceae
 13 K49 Bryopsis J. V. Lamouroux sp. Al-Yamani et al. (2014) mentioned B. hypnoides J. V. Lamouroux and B. plumosa (Hudson) C. Agardh, first record underpinned by molecular data in Kuwait
Phaeophyceae
 Acinetosporaceae
 14 K19 Acinetosporaceae G. Hamel ex Feldmann sp. Al-Yamani et al. (2014) mentioned F. mitchelliae and F. irregularis (Kützing) Hamel in Kuwait; according to DNA sequences, K19 is a different species
 15 K47, 48, 198, 216 Feldmannia mitchelliae (Harvey) H. -S. Kim Basionym: Ectocarpus mitchelliae Harvey, homotypic synonyms: Hincksia mitchelliae (Harvey) P. C. Silva, Giffordia mitchellae var. parva W. R. Taylor First record underpinned by molecular information in Kuwait, Al-Yamani et al. (2014) mentioned this taxon for Kuwait
 Ectocarpaceae
 16 K23 Ectocarpus subulatus Kützing Ectocarpus subulatus Kützing John and Al-Thani (2014) mentioned Ectocarpus siliculosus (Dillwyn) Lyngbye in the Gulf; E. subulatus is a new record for Kuwait and the Gulf, supported by molecular data
 Scytosiphonaceae
 17 K87 Colpomenia sinuosa Derbes et Solier Ulva sinuosus Mertens ex Roth Previously reported for Kuwait (Al-Adilah et al. 2020), further molecular information provided here
 18 K24 Iyengaria stellata (Børgesen) Børgesen Rosenvingea stellata Børgesen Previously reported for Kuwait (Santiañez et al. 2020), further molecular information provided here
 Sphacelariaceae
 19 K17, K71 Sphacelaria Lyngbye sp.
 20 K72, K84 Sphacelaria tribuloides Meneghin Sphacelaria reticulata Lyngbye Al-Yamani et al. (2014) mentioned this taxon for Kuwait; this work provides further molecular information
 Chordariaceae
 21 K20, 21, 22 Chordariaceae Greville sp. Al-Yamani et al. (2014) mentioned some sp. from Chordariaceae family under Cladosiphon, Myriactula, Nemacystus genus
 22 K26 Elachista stellaris Areschoug Areschougia stellaris (Areschoug) Meneghini New record underpinned by molecular information & the Gulf, this genus not mentioned before in Kuwait & the Gulf
 23 K69 Nemacystus decipiens (Suringar) Kuckuck Basionym Mesogloia decipiens Suringar Al-Yamani et al. (2014) mentioned this sp. in Kuwait, first record underpinned by molecular information in Kuwait
 Bachelotiaceae
 24 K70 Bachelotia (Bornet) Kuckuck ex Hamel sp. Kokabi and Yousefzadi (2015) mentioned B. antillarum (Grunow) Gerloff in the Gulf; this genus was not mentioned before in Kuwait, it is a new record for Kuwait, based on molecular analysis
Rhodophyta
 Acrochaetiales
 25 K34, 35, 109, 110 Acrochaetiales Feldmann sp. In the Gulf, Kokabi and Yousefzadi (2015) mentioned A. robustum Børgesen and A. savianum (Meneghini) Nägeli
 Acrochaetiaceae
 26 K13 Acrochaetium Nägeli & Cramer sp. John and Al-Thani (2014) mentioned A. robustum in the Gulf
 27 K101, 108 Acrochaetium secundatum (Lyngbye) Nägeli in Nägeli & Cramer Callithamnion daviesii var. secundatum Lyngbye New record for Kuwait & the Gulf underpinned by molecular information
 Ceramiaceae
 28 K82 Ceramium affine Setchell & N. L. Gardner No synonyms New record for Kuwait & the Gulf underpinned by molecular information
 29 K62 Ceramium Roth sp. Ceramium virgatum Roth Al-Yamani et al. (2014) mentioned C. luetzelburgii O. C. Schmidt in Kuwait
 Gelidiaceae
 30 K80 Gelidium pusillum var. pakistanicum Afaq-Husain & Shameel No synonyms New record for Kuwait & the Gulf underpinned by molecular information
 Rhodomelaceae
 31 K61 Polysiphonia Greville sp. Al-Yamani et al. (2014) mentioned P. brodiei (Dillwyn) Sprengel; P. coacta C. K. Tseng; P. denudate (Dillwyn) Greville ex Harvey; P. platycarpa Børgesen in Kuwait
 Erythrotrichiaceae
 32 K65 Erythrotrichiaceae G. M. Smith sp.
 33 K14, 29, 32, 33, 67 Erythrotrichia Areschoug sp. John and Al-Thani (2014) mentioned E. carnea (Dillwyn) J. Agardh; Kokabi and Yousefzadi (2015) mentioned E. irregularis Rosenvinge in the Gulf
 34 K36 Porphyrostromium Trevisan V. B. A. sp. New record underpinned by molecular information for Kuwait & the Gulf, this genus not mentioned before in Kuwait & the Gulf
 35 K27, 28, 103 Sahlingia subintegra (Rosenvinge) Kornmann Erythrocladia subintegra Rosenvinge First underpinned by molecular analysis in Kuwait and Al-Yamani et al. (2014) mentioned this species in Kuwait
 Spyridiaceae
 36 K66, 78 Spyridia Harvey sp. Al-Yamani et al. (2014) mentioned S.a. filamentosa (Wulfen) Harvey species in Kuwait
 Stylonemataceae
 37 K9 Chroodactylon Hansgirg sp. New record underpinned by molecular information for Kuwait. C. ornatum (C. Agardh) Basson has previously been mentioned by Al-Yamani et al. (2014)
 38 K12, 30, 32, 64, 104 Stylonema alsidii (Zanardini) K. Drew Bangia alsidii Zanardini John and Al-Thani (2014) mentioned this taxon for the Gulf, but it is a new record underpinned by molecular analysis for Kuwait
 Bangiaceae
 39 K118 Kuwaitiella rubra gen. et sp. nov. New record for Kuwait & Gulf underpinned by molecular information (Hasan et al. 2022)
 Callithamniaceae
 40 K63 Crouania Agardh sp. John and Al-Thani (2014) mentioned in the Gulf; C. attenuate J. Agardh, new record underpinned by molecular information for Kuwait; also, this genus was not reported before in Kuwait
 Delesseriaceae
 41 K68 Dasya caraibica Børgesen No synonyms New record underpinned by molecular analysis for the Gulf and Kuwait
 42 K81 Heterosiphonia Montagne [J. F.] sp. Al-Yamani et al. (2014) mentioned H. dendroidea Hollenberg; H. crispella (C. Agardh) M. J. Wynne
Table 3
Diversity of algal isolates obtained by Germling Emergence Method based on 90 algal cultures collected from Kuwaiti costal sites
No. Location sites Taxa

Chlorophyta Rhodophyta Phaeophyceae
1 Bubiyan Island - - Colpomenia sinuosa (K87)
2 Sabiya - - Ectocarpus subulatus (K23)
3 Failaka Island Ulva tepida (K54) Spyridia sp. (K66) Sphacelaria sp. (K71)
4 Doha Ulva tepida (K1, K3), Ulvella sp. (K39, K40), Ulvellaceae sp. (K43), Ulvella sp. (K44), Ulvellaceae sp. (K45, K46), Ulvella leptochaete (K50), Ulva ohnoi (K55), Rhizoclonium sp. (K58) Stylonema alsidii (K12)
5 Kuwait City Sharq - - -
6 Fintas - Sahlingia subintegra (K28), Stylonema alsidii (K31), Erythrotrichia sp. (K29, K32, K33), Porphyrostromium sp. (K36), Gelidium pusillum var. pakistanicum (K80), Heterosiphonia sp. (K81), Ceramium affine (K82) Iyengaria stellata (K24), Feldmannia mitchelliae s.I (K48)
7 Kubbar Island - - Feldmannia mitchelliae s.I (K198, K216)
8 Qaruh Island Ulva tepida (K51, K52) Crouania sp. (K63), Stylonema alsidii (K64), Erythrotrichiaceae sp. (K65) Sphacelaria tribuloides (K84)
9 Al-Zour Ulva tepida (K4, K5, K25), Planophilaceae sp. (K38, K41), Acrochaete sp. (K42), Cladophora laetevirens (K56), Cladophora gracilis (K57), Chaetomorpha sp. (K73, K74) Arcochaetium sp. (K13), Erythrotrichia sp. (K14, K33), Sahlingia subintegra (K27, K103), Stylonema alsidii (K30, K104), Acrochaetiales sp. (K34, K35, K109, K110), Acrochaetium secundatum (K101, K108), Dasya caraibica (K68) Acinetosporaceae sp. (K19), Chordariaceae sp. (K21), Chordariaceae sp. (K20), Chordariaceae sp. (K22), Feldmannia mitchelliae s.I (K47), Nemacystus decipiens (K69), Sphacelaria tribuloides (K72)
10 Khiran Bryopsis sp. (K49) Chroodactylon (K9), Polysiphonia sp. (K61), Spyridia sp. (K78), Kuwaitiella rubra gen. et sp. nov. (K118) Sphacelaria sp. (K17), Elachista stellaris (K26)
11 Umm Al-Maradim Island - - Bachelotia sp. (K70)
12 Nuwaiseeb - Ceramium sp. (K62) -

REFERENCES

Al-Adilah, H., Al-Bader, D., Elktob, M., Kosma, I., Kumari, P. & Küpper, F. C. 2021. Trace element concentrations in seaweeds of the Arabian Gulf identified by morphology and DNA barcodes. Bot. Mar. 64:327–338.
crossref
Al-Adilah, H., Peters, A. F., Al-Bader, D., Raab, A., Akhdhar, A., Feldmann, J. & Küpper, F. C. 2020. Iodine and fluorine concentrations in seaweeds of the Arabian Gulf identified by morphology and DNA barcodes. Bot. Mar. 63:509–519.
crossref
Alghunaim, A., Taqi, A., Al-Kandari, M. & Al-Said, T. 2019. Distribution and nature of Sargassum species in the Kuwait waters. J. Geosci. Environ. Prot. 7:53–59.

Al-Hasan, R. H. & Jones, W. E. 1989. Marine algal flora and sea grasses of the coast of Kuwait. J. Univ. Kuwait Sci. 16:289–341.

Al-Mutairi, N., Abahussain, A. & Al-Battay, A. 2014. Environmental assessment of water quality in Kuwait Bay. Int. J. Environ. Sci. Dev. 5:527–532.
crossref
Al-Said, T., Al-Ghunaim, A., Subba Rao, D. V., Al-Yamani, F., Al-Rifaie, K. & Al-Baz, A. 2017. Salinity-driven decadal changes in phytoplankton community in the NW Arabian Gulf of Kuwait. Environ. Monit. Assess. 189:268 pp.
crossref pmid pdf
Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25:3389–3402.
crossref pmid pmc
Al-Yamani, F. Y., Bishop, J., Ramadhan, E., Al-Husain, M. & Al-Ghadban, A. N. 2004. Oceanographic atlas of Kuwait waters. Kuwait Institute for Scientfic Research, Kuwait, 203 pp.

Al-Yamani, F. Y., Polikarpov, I., Al-Ghunaim, A. & Mikhaylova, T. 2014. Field guide of marine macroalgae (Chlorophyta, Rhodophyta, Phaeophyceae) of Kuwait. Kuwait Institute for Scientific Research, Kuwait, 190 pp.

Bartolo, A. G., Zammit, G., Peters, A. F. & Küpper, F. C. 2020. The current state of DNA barcoding of marcroalgae in the Mediterranean Sea: presently lacking but urgently required. Bot. Mar. 63:253–272.

Bolton, J. J. 1983. Ecoclinal variation in Ectocarpus siliculosus (Phaeophyceae) with respect to temperature growth optima and survival limits. Mar. Biol. 73:131–138.
crossref pdf
Broom, J. E. S., Nelson, W. A., Farr, T. J., Phillips, L. E. & Clayton, M. 2010. Relationships of the Porphyra (Bangiales, Rhodophyta) flora of the Falkland Islands: a molecular survey using rbc L and nSSU sequence data. Aust. Syst. Bot. 23:27–37.
crossref
Coelho, S. M., Scornet, D., Rousvoal, S., Peters, N. T., Dartevelle, L., Peters, A. F. & Cock, J. M. 2012. Ectocarpus: a model organism for the brown algae. Cold Spring Harb. Protoc. 2012. 193–198.
crossref pmid
Destombe, C. & Douglas, S. E. 1991. Rubisco spacer sequence divergence in the rhodophyte alga Gracilaria verrucosa and closely related species. Curr. Genet. 19:395–398.
crossref pmid pdf
De Vargas, C., Audic, S., Henry, N., Decelle, J., Mahé, F., Logares, R., Lara, E., Berney, C., Le Bescot, N., Probert, I., Carmichael, M., Poulain, J., Romac, S., Colin, S., Aury, J.-M., Bittner, L., Chaffron, S., Dunthorn, M., Engelen, S., Flegontova, O., Guidi, L., Horák, A., Jaillon, O., Lima-Mendez, G., Lukeš, J., Malviya, S., Morard, R., Mulot, M., Scalco, E., Siano, R., Vincent, F., Zingone, A., Dimier, C., Picheral, M., Searson, S., Kandels-Lewis, S., Acinas, S. G., Bork, P., Bowler, C., Gorsky, G., Grimsley, N., Hingamp, P., Iudicone, D., Not, F., Ogata, H., Pesant, S., Raes, J., Sieracki, M. E., Speich, S., Stemmann, L., Sunagawa, S., Weissenbach, J., Wincker, P.; Karsenti, E. & Tara Oceans Coordinators 2015. Eukaryotic plankton diversity in the sunlit ocean. Science. 348:1261605 pp.
crossref pmid
Dittami, S. M., Corre, E., Brillet-Guéguen, L., Lipinska, A.P., Pontoizeau, N., Aite, M., Avia, K., Caron, C., Cho, C.H., Collén, J., Cormier, A., Delage, L., Doubleau, S., Frioux, C., Gobet, A., González-Navarrete, I., Groisillier, A., Herv, C., Jollivet, D., KleinJan, H., Leblanc, C., Liu, X., Marie, D., Markov, G. V., Minoche, A. E., Monsoor, M., Pericard, P., Perrineau, M. M., Peters, A. F., Siegel, A., Siméon, A., Trottier, C., Yoon, H. S., Himmelbauer, H., Boyen, C. & Tonon, T. 2020a. The genome of Ectocarpus subulatus: a highly stress-tolerant brown alga. Mar. Genomics. 52:100740 pp.

Dittami, S. M., Peters, A. F., West, J. A., Cariou, T., KleinJan, H., Burgunter-Delamare, B., Prechoux, A., Egan, E. & Boyen, C. 2020b. Revisiting Australian Ectocarpus subulatus (Phaeophyceae) from the Hopkins River: distribution, abiotic environment, and associated microbiota. J. Phycol. 56:719–729.
crossref pmid
Edgar, R. C. 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 32:1792–1797.
crossref pmid pmc
Famà, P., Wysor, B., Kooistra, W. H. C. F. & Zuccarello, G. C. 2002. Molecular phylogeny of the genus Caulerpa (Caulerpales, Chlorophyta) inferred from chloroplast tuf A gene. J. Phycol. 38:1040–1050.
crossref
Fatemi, S. M. R., Ghavam Mostafavi, P., Rafiee, F. & Saeed Taheri, M. 2012. The study of seaweeds biomass from intertidal rocky shores of Qeshm Island, Persian Gulf. Int. J. Mar. Sci. Environ. 2:101–106.

Gachon, C. M. M., Strittmatter, M., Müller, D. G., Kleinteich, J. & Küpper, F. C. 2009. Detection of differential host susceptibility to the marine oomycete pathogen Eurychasma dicksonii by real-time PCR: not all algae are equal. Appl. Environ. Microbiol. 75:322–328.
crossref pmid pmc pdf
Hall, J. D., Fučíkov, K., Lo, C., Lewis, L. A. & Karol, K. G. 2010. An assessment of proposed DNA barcodes in freshwater green algae. Cryptogam. Algol. 31:529–555.

Han, K. Y., Graf, L., Reyes, C. P., Melkonian, B., Andersen, R. A., Yoon, H. S. & Melkonian, M. 2018. A re-investigation of Sarcinochrysis marina (Sarcinochrysidales, Pelagophyceae) from its type locality and the descriptions of Arachnochrysis, Pelagospilus, Sargassococcus and Sungminbooa genera nov. Protist. 169:79–106.
crossref pmid
Hasan, A. H., Van der Aa, P., Küpper, F. C., Al-Bader, D. & Peters, A. F. 2022. Kuwaitiella rubra gen. et sp. nov. (Bangiales, Rhodophyta), a new filamentous genus and species from the north-western Indian Ocean. Phycol. Res. 70:192–202.
crossref pdf
Hodge, F. J., Buchanan, J. & Zuccarello, G. C. 2010. Hybridization between the endemic brown algae Carpophyllum maschalocarpum and Carpophyllum angustifolium (Fucales): genetic and morphological evidence. Phycol. Res. 58:239–247.
crossref
Hoffmann, A. J. & Santelices, B. 1991. Banks of algal microscopic forms: hypotheses on their functioning and comparisons with seed banks. Mar. Ecol. Prog. Ser. 79:185–194.
crossref
John, D. M. 2012. Marine algae (seaweeds) associated with coral reefs of the Gulf. In : Riegl B. M., Purkis S. J., editors Coral Reefs of the Gulfs: Adaptation to Climate Extremes. Coral Reefs of the World. 3:Springer, Dordrecht, 170–186.

John, D. M. & Al-Thani, R. F. 2014. Benthic marine algae of the Arabian Gulf: a critical review and analysis of distribution and diversity patterns. Nova Hedwigia. 98:341–392.
crossref
Jones, D. 1986. A field guide to the seashores of Kuwait and the Arabian Gulf. University of Kuwait, Kuwait, 193 pp.

Kawai, H., Hanyuda, T., Draisma, S. G. A. & Müller, D. G. 2007. Molecular phylogeny of Discosporangium mesarthrocarpum (Phaeophyceae) with a reinstatement of the order discosporangiales. J. Phycol. 43:186–194.
crossref
Kirkendale, L., Saunders, G. W. & Winberg, P. 2013. A molecular survey of Ulva (Chlorophyta) in temperate Australia reveals enhanced levels of cosmopolitanism. J. Phycol. 49:69–81.
crossref pmid pdf
Kokabi, M. & Yousefzadi, M. 2015. Checklist of the marine macroalgae of Iran. Bot. Mar. 58:307–320.
crossref
Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol. Biol. Evol. 35:1547–1549.
crossref pmid pmc
Kunimoto, M., Kito, H., Kaminishi, Y., Mizukami, Y. & Murase, N. 1999. Molecular divergence of the SSU rRNA gene and internal transcribed spacer 1 in Porphyra yezoensis (Rhodophyta). J. Appl. Phycol. 11:211–216.

Küpper, F. C., Peters, A. F., Shewring, D. M., Sayer, M. D. J., Mystikou, A., Brown, H., Azzopardi, E., Dargent, O., Strittmatter, M., Brennan, D., Asensi, A. O., van West, P. & Wilce, R. T. 2016. Arctic marine phytobenthos of northern Baffin Island. J. Phycol. 52:532–549.
crossref pmid pmc pdf
Lane, C. E., Mayes, C., Druehl, L. D. & Saunders, G. W. 2006. A multi-gene molecular investigation of the kelp (Laminariales, Phaeophyceae) supports substantial taxonomic re-organization. J. Phycol. 42:493–512.
crossref
Lawton, R. J., Mata, L., de Nys, R. & Paul, N. A. 2013. Algal bioremediation of waste waters from land-based aquaculture using Ulva: selecting target species and strains. PLoS ONE. 8:e77344 pp.
crossref pmid pmc
Lewin, J. 1966. Silicon metabolism in diatoms. V. Germanium dioide, a specific inhibitor of diatom growth. Phycologia. 6:1–12.
crossref
Macreadie, P. I., Jarvis, J., Trevathan-Tackett, S. M. & Bellgrove, A. 2017. Seagrasses and macroalgae: importance, vulnerability and impacts. In : Phillips B. F., Pérez-Ramírez M., editors Climate Change Impacts on Fisheries and Aquaculture: A Global Analysis. Wiley-Blackwell, Oxford, 729–770.
crossref pdf
Martínez, B., Pato, L. S. & Rico, J. M. 2012. Nutrient uptake and growth responses of three intertidal macroalgae with perennial, opportunistic and summer-annual strategies. Aquat. Bot. 96:14–22.
crossref
Masakiyo, Y. & Shimada, S. 2014. Species diversity of the genus Ulva (Ulvophyceae, Chlorophyta) in Japanese waters, with special reference to Ulva tepida Masakiyo et S. Shimada sp. nov. Bull. Natl. Mus. Nat. Sci. Ser. B Bot. 40:1–13.

Mayakun, J. & Prathep, A. 2005. Seasonal variations in diversity and abundance of macroalgae at Samui Island, Surat Thani Province, Thailand. Songklanakarin J. Sci. Technol. 27:653–663.

Müller, D. G. & Ramírez, M. E. 1994. Filamentous brown algae from the Juan Fernandez Archipelago (Chile): contribution of laboratory culture techniques to a phytogeographic survey. Bot. Mar. 37:205–211.

Nishihara, G. N. & Terada, R. 2010. Spatial variations in nutrient supply to the red algae Eucheuma serra (J. Agardh) J. Agardh. Phycol. Res. 58:29–34.

Nybakken, J. W. 1993. Marine biology an ecological approach. Harper Collins College Publishers, NY, 10022 pp.

Pedersen, M. F., Borum, J. & Fotel, F. L. 2010. Phosphorus dynamics and limitation of fast- and slow-growing temperate seaweeds in Oslofjord, Norway. Mar. Ecol. Prog. Ser. 399:103–115.
crossref
Peters, A. F., Couceiro, L., Tsiamis, K., Küpper, F. C. & Valero, M. 2015. Barcoding of cryptic stages of marine brown algae isolated from incubated substratum reveals high diversity in Acinetosporaceae (Ectocarpales, Phaeophyceae). Cryptogam. Algol. 36:3–29.

Peters, A. F. & Ramírez, M. E. 2001. Molecular phylogeny of small brown algae, with special reference to the systematic position of Caepidium antarcticum (Adenocystaceae, Ectocarpales). Cryptogam. Algol. 22:187–200.
crossref
Peters, A. F., Scornet, D., Müller, D. G., Kloareg, B. & Cock, J. M. 2004. Inheritance of organelles in artificial hybrids of the isogamous multicellular chromist alga Ectocarpus siliculosus (Phaeophyceae). Eur. J. Phycol. 39:235–242.
crossref
Phillips, J. C. & Hurd, C. L. 2003. Nitrogen ecophysiology of intertidal seaweeds from New Zealand: N uptake, storage and utilisation in relation to shore position and season. Mar. Ecol. Prog. Ser. 264:31–48.
crossref
Pirian, K., Piri, K., Sohrabipour, J., Jahromi, S. T. & Blomster, J. 2016. Molecular and morphological characterisation of Ulva chaugulii, U. paschima and U. ohnoi (Ulvophyceae) from the Persian Gulf, Iran. Bot. Mar. 59:147–158.
crossref
Pokavanich, T. & Alosairi, Y. 2014. Summer flushing characteristics of Kuwait Bay. J. Coast. Res. 30:1066–1073.
crossref
Prathep, A., Wichachucherd, B. & Thongroy, P. 2007. Spatial and temporal variation in density and thallus morphology of Turbinaria ornata in Thailand. Aquat. Bot. 86:132–138.
crossref
Price, A. R. G., Vincent, L. P. A., Venkatachalam, A. J., Bolton, J. J. & Basson, P. W. 2006. Concordance between different measures of biodiversity in Indian Ocean macroalgae. Mar. Ecol. Prog. Ser. 319:85–91.
crossref
Provasoli, L. 1966. Media and prospects for the cultivation of marine algae. In : Watanabe A., Hattori A., editors Cult. Collect. Algae: Proc. US-Japan Conf. Japan Society of Plant Physiology, Hakone, 63–75.

Ramírez, M. E. & Müller, D. G. 1991. New records of benthic marine algae from Easter Island. Bot. Mar. 34:133–137.

Rizouli, A., Küpper, F. C., Louizidou, P., Mogg, A. O. M., Azzopardi, E., Sayer, M. D. J., Kawai, H., Hanyuda, T. & Peters, A. F. 2020. The minute alga Schizocladia ischiensis (Schizocladiophyceaase, Ochrophyta) isolated by germling emergence from 24 m depth of Rhodes (Greece). Diversity. 12:102 pp.
crossref
Santiañez, W. J. E., Al-Bader, D., West, J. A., Bolton, J. J. & Kogame, K. 2020. Status, morphology, and phylogenetic relationships of Iyengaria (Scytosiphonaceae, Phaeophyceae), a brown algal genus with a disjunct distribution in the Indian Ocean. Phycol. Res. 68:323–331.
crossref pdf
Saunders, G. W. 2005. Applying DNA barcoding to red macroalgae: a preliminary appraisal holds promise for future applications. Philos. Trans. R. Soc. Lond. B Biol. Sci. 360:1879–1888.
crossref pmid pmc pdf
Saunders, G. W. & Kucera, H. 2010. An evaluation of rbcL, tufA, UPA, LSU and ITS as DNA barcode markers for the marine green macroalgae. Cryptogam. Algol. 31:487–528.

Saunders, G. W. & McDevit, D. C. 2013. DNA barcoding unmasks overlooked diversity improving knowledge on the composition and origins of the Churchill algal flora. BMC Ecol. 13:9 pp.
crossref pmid pmc
Schoenrock, K. M., McHugh, T. A. & Krueger-Hadfield, S. A. 2021. Revisiting the ‘bank of microscopic forms’ in macroalgal-dominated ecosystems. J. Phycol. 57:14–29.
crossref pmid pdf
Siemer, B. L., Stam, W. T., Olsen, T. J. & Pedersen, P. M. 1998. Phylogenetic relationships of the brown algal orders Ectocarpales, Chordariales, Dictyosiphonales, and Tilopteridales (Phaeophyceae) based on RUBISCO large subunit and spacer sequences. J. Phycol. 34:1038–1048.
crossref
Silva, P. C., Basson, P. W. & Moe, R. L. 1996. Catalogue of the benthic marine algae of the Indian Ocean. 79:University California Press, Berkeley, CA, 1259 pp.

Starr, R. C. & Zeikus, J. A. 1993. UTEX: the culture collection of algae at the University of Texas at Austin 1993 List of cultures. J. Phycol. 29:1–106.
crossref
Tanaka, A., Uwai, S., Nelson, W. & Kawai, H. 2010. Phaeophysema gen. nov. and Vimineoleathesia gen. nov., new brown algal genera for the minute Japanese members of the genus Leathesia . Eur. J. Phycol. 45:107–115.
crossref
Tarakhovskaya, E. R., Kang, E. J., Kim, K. Y. & Garbary, D. J. 2012. Effect of GeO2 on embryo development and photosynthesis in Fucus vesiculosus (Phaeophyceae). Algae. 27:125–134.
crossref
Uddin, S., Al Ghadban, A. N. & Khabbaz, A. 2011. Localized hyper saline waters in Arabian Gulf from desalination activity: an example from South Kuwait. Environ. Monit. Asses. 181:587–594.
crossref pmid pdf
West, J. A., Loiseaux-De Goër, S. & Zuccarello, G. C. 2012. Upright Erythropeltidales (Rhodophyta) in Brittany, France and description of a new species, Erythrotrichia longistipitata . Cah. Biol. Mar. 53:255–270.

White, T. J., Bruns, T., Lee, S. & Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In : Innis M. A., Gelfand D. H., Sninsky J. J., White T. J., editors PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, CA, 315–322.
crossref
Wolf, M. 2012. Molecular and morphological investigations on seaweed biodiversity and alien introductions in the Adriatic Sea (Mediterranean, Italy). Ph.D. dissertation. Universit Degli Studi Di padova Dipartimento Di Biologia, Padova, Italy, 183 pp.

Wynne, M. J., Kamiya, M., West, J.A., Loiseaux-de Goër, S., Lim, P.-E., Sade, A., Russell, H. & Küpper, F. C. 2020. Morphological and molecular evidence for the recognition of Hypoglossum sabahense sp. nov. (Delesseriaceae, Rhodophyta) from Sabah, Malaysia. Algae. 35:157–165.
crossref pdf
Yoon, H. S., Hackett, J. D. & Bhattacharya, D. 2002. A single origin of the peridinin- and fucoxanthin-containing plastids in dinoflagellates through tertiary endosymbiosis. Proc. Natl. Acad. Sci. U. S. A. 99:11724–11729.
crossref pmid pmc
Zuccarello, G. C., Yoon, H. S., Kim, H., Sun, L., de Gor, S. & West, J. A. 2011. Molecular phylogeny of the upright erythropeltidales (Compsopogonophyceae, Rhodophyta): multiple cryptic lineages of Erythrotrichia carnea . J. Phycol. 47:627–637.
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