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Algae > Volume 30(4); 2015 > Article
Algae 2015;30(4): 313-323. doi: https://doi.org/10.4490/algae.2015.30.4.313
Evaluation of phlorofucofuroeckol-A isolated from Ecklonia cava (Phaeophyta) on anti-lipid peroxidation in vitro and in vivo
Ji-Hyeok Lee1, Ju-Young Ko1, Jae-Young Oh1, Eun-A Kim1, Chul-Young Kim2 and You-Jin Jeon1,*

1Department of Marine Life Science, Jeju National University, Jeju 63243, Korea
2Natural Product Research Center, Hanyang University, Ansan 15588, Korea
*Corresponding Author  Email: youjin2014@gmail.com
Lipid peroxidation means the oxidative degradation of lipids. The process from the cell membrane lipids in an organism is generated by free radicals, and result in cell damage. Phlorotannins, well-known marine brown algal polyphenols, have been utilized in functional food supplements as well as in medicine supplements to serve a variety of purposes. In this study, we assessed the potential anti-lipid peroxidation activity of phlorofucofuroeckol-A (PFF-A), one of the phlorotannins, isolated from Ecklonia cava by centrifugal partition chromatography in 2,2-azobis (2-amidinopropane) dihydrochloride (AAPH)-stimulated Vero cells and zebrafish system. PFF-A showed the strongest scavenging activity against alkyl radicals of all other reactive oxygen species (ROS) and exhibited a strong protective effect against ROS and a significantly strong inhibited of malondialdehyde in AAPH-stimulated Vero cells. The apoptotic bodies and pro-apoptotic proteins Bax and caspase-3, which were induced by AAPH, were strongly inhibited by PFF-A in a dose-dependent manner and expression of Bcl-xL, an anti-apoptotic protein, was induced. In the AAPH-stimulated zebrafish model, additionally PFF-A significantly inhibited ROS and cell death, as well as exhibited a strong protective effect against lipid peroxidation. Therefore, these results suggest that PFF-A has excellent protective effects against ROS and lipid peroxidation induced by AAPH in both an in vitro Vero cell model and an in vivo zebrafish model.
Key words: AAPH; Ecklonia cava; lipid peroxidation; phlorofucofuroeckol-A; Vero cell model; zebrafish model

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