In vitro antiviral activity of dieckol and phlorofucofuroeckol-A isolated from edible brown alga Eisenia bicyclis against murine norovirus

doi:2015.30.3.241

Algae > Volume 30(3); 2015 > Article

Algae 2015;30(3): 241-246. doi: https://doi.org/10.4490/algae.2015.30.3.241

Sung-Hwan Eom¹, Sun-Young Moon², Dae-Sung Lee³, Hyo-Jung Kim⁴, Kunbawui Park⁵, Eun-Woo Lee⁶, Tae Hoon Kim⁷, Yong-Hyun Chung⁸, Myung-Suk Lee⁹ and Young-Mog Kim^4,*

¹Korea Food Research Institute, Sungnam 463-746, Korea
²Department of Interdisciplinary Program of Biomedical Engineering, Pukyong National University, Busan 608-737, Korea
³National Marine Biodiversity Institutes of Korea, Seocheon 325-902, Korea
⁴Department of Food Science and Technology, Pukyong National University, Busan 608-737, Korea
⁵Food Safety Research Division, National Fisheries Research & Development Institute, Busan 619-705, Korea
⁶Department of Life Science and Biotechnology, Dongeui University, Busan 614-714, Korea
⁷Department of Food Science and Biotechnology, Daegu University, Gyeongsan 712-714, Korea
⁸Department of Ecological Engineering, Pukyong National University, Busan 608-737, Korea
⁹Department of Microbiology, Pukyong National University, Busan 608-737, Korea

*Corresponding Author

Email: ymkim@pknu.ac.kr

ABSTRACT

This research was conducted to develop effective and safe marine-derived antiviral compounds against norovirus. The ethyl acetate (EtOAc)-extract from Eisenia bicyclis exhibited strong antiviral activity against murine norovirus (MNV) as a norovirus surrogate. Among the phlorotannins from E. bicyclis, dieckol (DE) and phlorofucofuroeckol-A (PFF) were known to possess the strongest antibacterial activity. In this study, DE and PFF were evaluated for antiviral activity against MNV. DE and PFF exhibited strong anti-MNV activity with 50% effective concentration (EC50) of 0.9 μM. However, PFF exhibited more effective antiviral activity against MNV with higher selective index (668.87) than that of DE (550.60), due to its lower cell toxicity against RAW 264.7. This is the first report on the anti-MNV activity of phlorotannins from seaweed. The results obtained in this study suggest that the phlorotannins could be used as a potential source of natural antiviral agents.

Key words: antiviral activity; Eisenia bicyclis; murine norovirus; phlorofucofuroeckol-A; phlorotannins