Regeneration Studies in Grateloupia filicina (J.V. Lamouroux) C. Agardh – An Important Carrageenophyte and Edible Seaweed

doi:2009.24.3.163

Algae > Volume 24(3); 2009 > Article

Algae 2009;24(3): 163-168. doi: https://doi.org/10.4490/algae.2009.24.3.163

Pooja Baweja and Dinabandhu Sahoo^*

Marine Biotechnology Laboratory, Department of Botany, University of Delhi, Delhi – 110007, India

*Corresponding Author

Email: dbsahoo@hotmail.com

ABSTRACT

Grateloupia filicina (J.V. Lamouroux) C. Agardh (Halymeniaceae, Cryptonemiales, Rhodophyta) is an edible seaweed as well as an important source of carrageenan. In the present study, attempt has been made to develop a suitable protocol for effective regeneration of the seaweed and the rapid multiplication of the desired varieties. The young upright thallus of G. filicina was grown in axenic culture using both solid and liquid media. The various media tested were f/2, Provasoli’s Enriched Seawater (PES) and Enriched Seawater (ESW). The effect of glycerol (as a carbon source) and various plant growth regulators i.e. auxin (NAA) and cytokinins (Kinetin and BA) were tested. Although, regeneration of young thalli was observed from the cut ends in all the media, better growth was found in f/2, PES, f/2 (0.5% Glycerol), f/2 (NAA 10?5 M) and f/2 (BA 10?6 M). On the other hand callusing was observed only in solid media supplemented with low concentration of Glycerol (0.5%) in f/2, NAA 10?5 M in f/2, PES and BA 10?5 M in f/2. Young thalli were developed from the callus sub culture after 40 days of inoculation.

Key words: callusing, carrageenophyte, Grateloupia filicina, regeneration