| Home | E-Submission | Sitemap | Contact Us |  
top_img
Algae > Volume 18(1); 2003 > Article
Algae 2003;18(1): 65-69. doi: https://doi.org/10.4490/ALGAE.2003.18.1.065
A Simple Method for DNA Extraction from Red Algae
Yoo Kyung Lee, Hong Kum Lee
National Research Lab for Marine Microbial Diversity, Korea Ocean Research and Development institute
ABSTRACT
A simple method is described for extracting DNA from marine red algae from which it has been difficult to isolate DNA because of their polysaccharides and phenolic compounds. In this DNA extraction method, the red algae were lysed mechanically by freezing and grinding in liquid nitrogen, and chemically by SDS. Then, the DNA was purified in phenol/chloroform, and precipitated in isopropanol. This method was applied to Antithamnion sparsum, Campylaephora crassa, Gelidium amansii, Griffithsia japonica, Polysiphonia morrowii, Porphyra yezoensis, and Symphyocladia latiuscula. The DNA extracted by this method is high yield and high quality for molecular analyses, such as PCR, sequencing, restriction enzyme digestion, and genomic DNA library construction. This method is reproducible, simple, and fast for routine DNA extraction from red algae.
Key words: Antithamnion, Campylaephora, DNA extraction, Gelidium, Griffithsia, Polysiphonia, Porphyra, Symphyocladia


TOOLS
PDF Links  PDF Links
Full text via DOI  Full text via DOI
Download Citation  Download Citation
Share:      
METRICS
1
Crossref
0
Scopus
6,960
View
527
Download
Related articles
A Simple Method for RNA Extraction from Marine Macroalgae   1997 ;12(1)
A Simple Method of Seaweed Axenic Culture   1989 ;4(2)
Editorial Office
[14348] A-1716, Gwangmyeong Trade Center, 72 Iljik-ro Gwangmyeong-si. Gyeonggi-do, Korea
Tel: +82-2-899-5980  Fax: +82-2-899-5922    E-mail: editalgae@gmail.com
About |  Browse Articles |  Current Issue |  For Authors and Reviewers
Copyright © The Korean Society of Phycology.                 Developed in M2PI