Activity of Chlorella vulgaris Associated by Escherichia coli W3110 on Removal of Total Organic Carbon in Continuous River Water Flow System
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Surk-Key Kong, Nakajima Toshiuki
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Department of Environmental Engineering, Joongbu University, Department of Biology and Earth Sciences, College of Theological Sciences, Ehime University
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ABSTRACT |
We investigated the association of Chlorella vulgaris and E. coli W3110 in removal of total organic carbon with the lab-scaled continuous river water flow system (CRWFS). Artificial synthetic wastewater was applied at two levels of organic carbon concentration; 1,335 ㎎?l-1 in the treatment (T)-1 and 267 ㎎?l-1 in T-2. The highest densities of C. vulgaris were 8.3×106 cells?ml-1 in T-1 and 6.9×106 cells?ml-1 in T-2. The maximum densities of E. coli W3110 were 2.0×108 colony forming unit (CFU)?ml-1 in T-1 and 3.9×108 CFU?ml-1 in T-2. The densities increased during the first 11 days in T-1 and 4 days in T-2, and decreased rapidly till 35th day, then increased slightly afterwards. This trend was prominent in T-2. It was implied that wider range of untrients was required in the growth of heterotrophic bacteria in T-2 than in T-1. The algal biomass should be increased effectively for the successful removal of organic carbon.
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Key words:
algal biomass, association of algae and bacterium, Chlorella vulgaris, Escherichia coli W3110 |
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