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Algae 2000;15(4): 307-314. |
| Quantification of DSP Toxins in the Mussels of the Jinhae Bay by Fluorometric HPLC Analysis and Protein Phosphatase Inhibition Assay
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| Duck Kyun Ahn, Young Shik Park, Jong-Gyu Park, Jong Soo Lee, Jin Ae Lee
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| ABSTRACT |
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The diarrhetic shellfish toxins in the midgut of mussels (Mytilus edulis) were quanitified by fluorometric high performance liquid chromatography analysis (HPLC-FD) and protein phosphatase inhibition assay (PPIA) during the summer 1999 at the 4 stations in the Jinhae Bay, Korea. Dinophysis acuminata and D. fortii were identified in the present study and the cell density of Dinophysis was up to 1,650 cells/ l. Dinophysis acuminata was the dominant species with the relative abundance ranging from 94% to 100%. The contents of okadaic acid (OA) and dinophysistoxin-1 (DTX-1) were not detectable in all samples by HPLC-FD analysis. However, total DSP in the mussel varied from 13.5 to 96.7 ng/g midgut gland by PPIA. The detection limit of the PPIA was 20 pg/g midgut gland, and the assay will be useful for routine monitoring of DSP toxins at trace level. The cell density of Dinophysis in the water column was not responsible for the OA concentrations in the mussels in the present study.
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