| Home | E-Submission | Sitemap | Contact Us |  
Algae > Volume 12(2); 1997 > Article
Algae 1997;12(2): 99-104.
Preparation of Cell Wall Degrading Enzymes from Marine Herbivores for Algal Protoplast Isolation 1. Cell Wall Degradation and Isolation of Protoplasts from a Marine Red Alga Porphyra okamurae Ueda by Enzyme Extracts of Abalone (Nordotis discus (Reeve))
Jae-Suk Choi, Chang-Geun Choi, Young-Dae Kim, hyung-Geun Kim, Bang-Ook Jun
Department of Biology and Faculty of Marine Bioscience and Technology, Kangnung National University, Kangnung Hatchery, National Fisheries Research and Development Agency
In order to develop algal cell wall degrading enzymes, for the protoplast colation, from marine herbivores native to Korean coast, the acetone powder extract of abalone (Nordotis discus (Reeve)) was prepared. Protoplasts were isolated from a marine red alga Porphyra okamurae Ueda using prepared acetone powder extracts of abalone and commercial cellulase mixture. The optimal condition for the release of protoplasts was found to be a combination of 1% prepared abalone acetone powder and 3% cellulase in 0.5M mannitol at pH 6.0. The acetone powder extract of abalone produced similar or better protoplast yield compared to the commercial abalone acetone powder.
Key words: balone, algal protoplast, cell wall degrading enzyme, Porphyra okamurae

PDF Links  PDF Links
Full text via DOI  Full text via DOI
Download Citation  Download Citation
Related article
Editorial Office
[14348] A-1716, Gwangmyeong Trade Center, 72 Iljik-ro Gwangmyeong-si. Gyeonggi-do, Korea
Tel: +82-2-899-5980  Fax: +82-2-899-5922    E-mail: editalgae@gmail.com
About |  Browse Articles |  Current Issue |  For Authors and Reviewers
Copyright © The Korean Society of Phycology.                 Developed in M2PI