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Algae 1995;10(2): 117-119. |
| A Simple and Rapid Method for Isolation of Total DNA from Free-Living Conchocelis of Porphyra (Bangiales, Rhodophyta)
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| Jong-Ahm Shin, Shigeto Kiyokawa, Yumi Kidachi, Akio Miura
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Department of Bioscience and Biotechnology, Faculty of Engineering
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| ABSTRACT |
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We describe more simple, inexpensive and rapid method for isolation of total DNA from free-living conchocelis of some Porphyras than the previous methods requiring quantities of fresh tissue and lots of time, labor and cost. The method yields sufficient quantities of good-quality ANA from a small amount of conchocelis tissue without liquid nitrogen treatments, hexadecyltrimetylammonium bromide (CTAB) treatments and phenol extraction.
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| Key words:
conchocelis DNA isolation, Porphyra, Rhodophyta |
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