| Home | E-Submission | Sitemap | Contact Us |  
top_img
Algae > Volume 4(1); 1989 > Article
Algae 1989;4(1): 63-67.
Studies on Nitrate Reductase in Ulva pertusa 1. In vivo Nitrate Reductase Activity
Bang-Ook Jun, Dae-Hwan Kim
Department of Biology, Kangreung National University
ABSTRACT
The applicability of in vivo nitrate reductase assay and effects of several factors influencing enzyme induction in Ulva pertusa were investigated. Four percent propanol was most suitable for the in vivo assay, and starvation of 3 days are prerequisite for induction study. For the induction of nitrate reductase both light and nitrate were required. Glucose could not substitute for light. DCMU completely inhibited, whereas 2,4-dinitrophenol, cyanide and azide hardly affected enzyme induction. These results suggest the possibility that reducing power required for the induction of nitrate reductase is NADPH. Tungstate inhibited increment of nitrate reductase activity probably by competition with molybdate required for enzyme activity. Ammonium, ion and cycloheximide repressed enzyme induction.
Key words: In vivo assay, nitrate reductase, Ulva pertusa.


TOOLS
PDF Links  PDF Links
Full text via DOI  Full text via DOI
Download Citation  Download Citation
CrossRef TDM  CrossRef TDM
Share:      
METRICS
1,066
View
6
Download
Related article
On the Nitrate Uptake in Ulva pertusa Kjellman   1994 ;9(2)
Editorial Office
[14348] A-1716, Gwangmyeong Trade Center, 72 Iljik-ro Gwangmyeong-si. Gyeonggi-do, Korea
Tel: +82-2-899-5980  Fax: +82-2-899-5922    E-mail: editalgae@gmail.com
About |  Browse Articles |  Current Issue |  For Authors and Reviewers
Copyright © The Korean Society of Phycology.                 Developed in M2PI