Cold-tolerant strain of Haematococcus pluvialis (Haematococcaceae, Chlorophyta) from Blomstrandhalvøya (Svalbard)

doi:2013.28.2.185

Algae > Volume 28(2); 2013 > Article

Algae 2013;28(2): 185-192. doi: https://doi.org/10.4490/algae.2013.28.2.185

Tatyana A. Klochkova¹, Min Seok Kwak², Jong Won Han², Taizo Motomura³, Chikako Nagasato³ and Gwang Hoon Kim²^,*

¹Kamchatka State Technical University (KamchatGTU), Petropavlovsk-Kamchatsky, Klyuchevskaya 35, 683003, Russia
²Department of Biology, Kongju National University, Kongju 314-701, Korea
³Muroran Marine Station, Field Science Centre for Northern Biosphere, Hokkaido University, Muroran 051-0003, Japan

*Corresponding Author

Email: ghkim@kongju.ac.kr

ABSTRACT

A new cold-adapted Arctic strain of Haematococcus pluvialis from Blomstrandhalvøya Island (Svalbard) is described. This strain is predominantly always in non-motile palmelloid stage. Transmission electron microscopy showed the presence of very thick cell wall and abundant lipid vesicles in the palmelloids, including red and green cells. The external morphology of the non-motile palmelloid and motile bi-flagellated cells of our strain is similar to H. pluvialis; however it differs from H. pluvialis in physiology. Our strain is adapted to live and produce astaxanthin in the low temperature (4- 10°C), whilst the usual growth temperature for H. pluvialis is between 20-27°C. Phylogenetic analysis based on 18S rRNA gene data showed that our strain nested within the Haematococcus group, forming a sister relationship to H. lacustris and H. pluvialis, which are considered synonymous. Therefore, we identified our Arctic strain as H. pluvialis.

Key words: astaxanthin; Blomstrandhalv