Antioxidant activity and cell protective effect of loliolide isolated from Sargassum ringgoldianum subsp. coreanum

doi:2011.26.2.201

Algae > Volume 26(2); 2011 > Article

Algae 2011;26(2): 201-208. doi: https://doi.org/10.4490/algae.2011.26.2.201

Xiudong Yang¹, Min-Cheol Kang¹, Ki-Wan Lee¹, Sung-Myung Kang¹, Won-Woo¹ Lee¹ and You-Jin Jeon¹

Department of Marine Life Science, Jeju National University, Jeju 690-756, Korea

*Corresponding Author

Email: youjinj@jejunu.ac.kr

ABSTRACT

A monoterpene lactone, loliolide was successively isolated from brown seaweed, Sargassum ringgoldianum subsp. coreanum using column chromatography and high-performance liquid chromatography. The structure of the isolated compound was determined by comparison of EIMS and NMR spectral data with the previously published data. The antioxidant activities of loliolide were also evaluated by DPPH and H2O2 radical and intracellular reactive oxygen species scavenging assays, and it showed moderate activities in all antioxidant assays. Furthermore, loliolide was found to exert positive dose-dependent effects in the protective effects against H2O2-induced cell damage which were determined via MTT, Hoechst staining assays and cell cycle analysis. This is the first study on chemical constituent of this seaweed according to our best knowledge. These results clearly indicated that loliolide isolated from Sargassum ringgoldianum subsp. coreanum can protect the cells against H2O2-induced cell damage or apoptosis.

Key words: antioxidant; cell cycle; cell protective; Loliolide; Sargassum ringgoldianum